Real-time PCR - detection of a specific species
Services offered:
- Single species detection from environmental DNA, barcoding DNA
- Research conducted to verify the occurrence of specific protected species
- Inventories of extensive areas subject to protection and areas of potential impact of planned investments in relation to the species occurrence and the state of its population
- Inventory of invasive alien species (IAS)
- Designing appropriate kits for the detection of specific species based on eDNA analysis
Do you need environmental eDNA analysis?
The only thing you need to make it happen is reliable professionals.
Real-time PCR is a modern method of species identification, based on the amplification of DNA fragments in real time, which enables precise detection of organisms in the examined environment. The use of this technique in eDNA analysis allows for the detection of specific protected species, as well as invasive alien species (IAS), which can be a threat to the Polish and EU environment.
An important asset of this method is its ability to identify species valuable in the environment, which allows for early identification of potential environmental threats resulting from investment plans. The use of molecular analysis of environmental DNA enables the detection of protected species from malacofauna, ichthyofauna and herpetofauna.
Environmental studies, such as analysis of water samples taken from sections of rivers, lakes, reservoirs or wetlands, are carried out in our laboratory, which is an alternative to traditional, time-consuming field methods. Thanks to the use of real-time PCR, it is possible to quickly obtain results regarding the presence of species in a given area, without the need for long-term field studies.
Examples of completed projects by the eDNA Laboratory of LNRA
The State Forest, forest district Dąbrowa
Verification of the presence of the marsh minnow (Rhynchocypris percnurus) within the boundaries of the planned nature reserve "Dolina rzeki Mątawy" (Kuyavian-Pomeranian Voivodeship) using real-time PCR technique
PPT Consult LLC.
Species identification of the thick-shelled river mussel (Unio crassus) in the section of the river Brda in Nadolna Karczma (Kuyavian-Pomeranian Voivodeship) using molecular research methods (barcoding) to verify species affiliation.
Regional Directors for Environmental Protection in Warsaw
Study of the species composition of fish in the river Bug using modern research methods (metabarcoding – nanopore sequencing) within the project entitled “Environmental monitoring presenting the assessment of the state of preservation of individual protected objects of the Natura 2000 Ostoja Nadbużańska PLH140011 area – natural habitats, fish”
EKOSTUDIUM
Conducting an inventory and ichthyological valuation of mining areas at Rejewskiego Street in Bydgoszcz, along with the development of results and analysis of the impact of the planned land development on the aquatic environment
Selected services
DNA barcoding
Metabarcoding eDNA
